SP100 Knockout cell line (THP-1)

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SP100 Knockout cell line (THP-1) Order

SP100 Knockout cell line (THP-1)
Catalog#
YKO-HT0261
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
SP100 Knockout cell line (THP-1)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
SP100 Knockout cell line (THP-1)
Species
Human
Gene Name
SP100
Gene ID
6672
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 3 (based on transcript SP100-202)
Validation: PCR+Sanger Sequencing

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the SP100 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the THP-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, SP100 gene knockout SP100 Knockout cell line (THP-1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
SP100 Knockout cell line (THP-1)
SP100 Knockout cell line (THP-1)
Catalog#
YKO-HT0261
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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Wild-type Cell Lines

Gene Knockout kit

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  • SP100 Knockout cell line (U-2 OS)

    Organism: Human
    Gene Name: SP100
    Gene ID: 6672
    Size: 1*10^6
    Catalog#: YKO-HT13340
    Request a Quote More details

  • SP100 Knockout cell line (Jurkat)

    Organism: Human
    Gene Name: SP100
    Gene ID: 6672
    Size: 1*10^6
    Catalog#: YKO-HT11786
    Request a Quote More details

  • SP100 Knockout cell line (Hep G2)

    Organism: Human
    Gene Name: SP100
    Gene ID: 6672
    Size: 1*10^6
    Catalog#: YKO-HT6681
    Request a Quote More details

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